If you require support or assistance when using our kit, please contact firstname.lastname@example.org and we will work rapidly to help you find a solution.
FREQUENTLY ASKED QUESTIONS (FAQ)
The washing system plays an important role in the quality of the final results:
- Fill the wells completely at each wash
- Tap hard after the last wash to remove all fluid.
- Avoid drying of wells between washings.
- Do not scrape the bottom of the wells during the washing steps, which could remove the antigen/antibody complexes.
- If using a manual wash method, overflowing of wells is not a problem. However, after the first wash it is important to empty the wells quickly to avoid contamination between wells.
- The cleanliness of the wash system may affect the quality of the results. Bacterial contamination of a tube or pump may arise if the system is never washed. We suggest that you pay particular attention to the cleanliness of the washing apparatus, regularly decontaminating the washer with a suitable detergent.
All available washing methods are compatible with ELISA kits:
- squeeze bottle
- manual comb system
- automatic washers
Please contact Elabscience or your sales representative immediately. Be sure to provide the following information:
- Contact details of your lab
- Kit product code and batch number
- OD results of the plate(s), indicating the position of the positive and negative controls
- The clinical history of the animals tested (ex. clinical signs; from an infected or disease-free herd).
- Detailed operational information
When comparing results obtained by methods based on different principles (ex. LFA vs. VNT vs. ELISA vs. CFT vs.), it is practically impossible to obtain perfect test agreement, unless very strongly positive and perfectly negative samples are used.
This is also true when comparing cELISA and iELISA results. The polyclonal nature of serum means that it contains populations of IgGs with different affinities for different antigens, each antigen with many epitopes. Each technique detects these populations to a different extent. Sera close to the cut-off will show more discordant results, as they contain more limited quantities of each type of antibody.